[PDF] Mycobacteria Protocols

Mycobacteria Protocols PDF
Author: Tanya Parish
Publisher: Humana Press
ISBN: 9781493924493
Size: 60.96 MB
Format: PDF, Docs
Category : Science
Languages : un
Pages : 416
View: 7585

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Mycobacteria Protocols

by Tanya Parish, release date 2015-03-17, Mycobacteria Protocols Books available in PDF, EPUB, Mobi Format. Download Mycobacteria Protocols books, Updated and revised, this thorough volume provides a selection of the newest methods, as well as some of the basic methods required for a mycobacterial research laboratory. Mycobacteria Protocols, Third Edition guides readers through fractionation and analysis of macromolecules, from nucleic acids to proteins, complex lipids, and metabolites. Detailed and comprehensive protocols are provided for protein and lipid/glycolipid analysis using well-established methods; these are now complemented by a metabolomics chapter in which the complement of metabolites can be profiled. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Mycobacteria Protocols, Third Edition will be a resource both to those working in the field and to newcomers.




[PDF] Mycobacteria Protocols

Mycobacteria Protocols PDF
Author: Tanya Parish
Publisher: Humana Press
ISBN: 9781588298898
Size: 48.47 MB
Format: PDF
Category : Science
Languages : un
Pages : 456
View: 3130

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Mycobacteria Protocols

by Tanya Parish, release date 2008-10-20, Mycobacteria Protocols Books available in PDF, EPUB, Mobi Format. Download Mycobacteria Protocols books, Due to the rising threat of tuberculosis and other mycobacterial infections, methods to study the biology of the mycobacteria and to improve diagnostic, therapeutic and preventative reagents are still very much in need. Mycobacteria Protocols, Second Edition updates and refines the methods of the well-received first edition while adding newly developed methods culled from the most cutting-edge research in the field. Ranging from the basics of sub-cellular fractionation to advanced methods using specialized growth conditions and whole genome, transcriptome and proteome analysis, the chapters conform to the Methods in Molecular BiologyTM series format, providing step-by-step laboratory protocols, lists of necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Mycobacteria Protocols, Second Edition is the perfect resource to promote and stimulate further research into these intriguing, important and most fractious of bacteria.




[PDF] Mycobacterium Tuberculosis Protocols

Mycobacterium Tuberculosis Protocols PDF
Author: Tanya Parish
Publisher: Springer Science & Business Media
ISBN: 1592591477
Size: 12.24 MB
Format: PDF
Category : Medical
Languages : en
Pages : 419
View: 4314

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Mycobacterium Tuberculosis Protocols

by Tanya Parish, release date 2001, Mycobacterium Tuberculosis Protocols Books available in PDF, EPUB, Mobi Format. Download Mycobacterium Tuberculosis Protocols books, With one-third of the world's population infected with Mycobacterium tuberculosis, over two million people a year dying from tuberculosis, and the appearance of multidrug-resistant strains, the need to understand the biology of M. tuberculosis, and so to develop new interventions, has become acute. In Mycobacterium tuberculosis Protocols, leading investigators with extensive practical knowledge and experience describe their best methods for studying this dangerous pathogen. Packed with step-by-step instructions to ensure successful results, these methods range from basic handling techniques to the application of functional genomics. These molecular techniques are suitable for research in genetics, biochemistry, microbiology, cell biology, epidemiology, and diagnostics, and are at the forefront of biological research as a whole, as well as in focused M. tuberculosis research. Highlights include methods for the basic safety and culture of M. tuberculosis, fractionation of the bacterium (nucleic acids, lipids, culture filtrate, and capsule), the analysis of gene expression (start-site mapping, real-time PCR, microarrays, and proteomics), the growth of the bacterium in macrophages and low oxygen, cytological analysis of the bacteria, and diagnostics. Highly practical and accessible, Mycobacterium tuberculosis Protocols utilizes advanced functional genomics and mutagenesis methodologies to provide both experimental and clinical investigators all the powerful techniques needed to illuminate the molecular biology of tuberculosis and its interactions with host cells, and so drive work on the wide variety of emerging therapeutic opportunities.




[PDF] Mycobacteria

Mycobacteria PDF
Author: C. Ratledge
Publisher: John Wiley & Sons
ISBN: 1444311441
Size: 78.81 MB
Format: PDF, Mobi
Category : Science
Languages : en
Pages : 416
View: 2635

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Mycobacteria

by C. Ratledge, release date 2009-04-01, Mycobacteria Books available in PDF, EPUB, Mobi Format. Download Mycobacteria books, Tuberculosis continues to kills more people than any other single infective agent. The resurgence of the disease in many countries has produced a heightened awareness of the threat posed by mycobacterial infections. At the same time, there has been an explosion of knowledge of the fundamental properties of mycobacteria, most notably the determination of the complete genome sequence of Mycobacterium tuberculosis. This book provides an up-to-date account of these developments in the molecular biology and immunology of mycobacteria, coupled with allied advances of a more applied nature, such as the use of molecular techniques for diagnosis and epidemiological investigations. With chapters contributed from an international team of experts, it will not only be an essential reference text for the expanding mycobacterial research community, but also will find a prominent place on the shelves of clinicians, infectious disease and public health specialists, diagnostic laboratories, postgraduate students and indeed anyone concerned with the management and investigation of outbreaks of tuberculosis. Comprehensively covers recent advances in the molecular biology of the mycobacteria. First book to be published on this subject since the publishing of the complete genome for the tubercle bacillus (M. tuberculosis). Coincides with a worldwide resurgence of tuberculosis.




[PDF] Riboswitches As Targets And Tools

Riboswitches as Targets and Tools PDF
Author:
Publisher: Academic Press
ISBN: 0128013362
Size: 61.51 MB
Format: PDF, ePub, Docs
Category : Science
Languages : en
Pages : 444
View: 2292

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Riboswitches As Targets And Tools

by , release date 2015-01-12, Riboswitches As Targets And Tools Books available in PDF, EPUB, Mobi Format. Download Riboswitches As Targets And Tools books, This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in riboswitches as targets and tools and contains sections on such topics as constructing and optimizing artificial riboswitches, live cell imaging and intracellular sensors with artificial riboswitches, conditional control of gene expression with artificial riboswitches, using artificial riboswitches for protein evolution and pathway optimization, and anti-riboswitch drug screens. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in riboswitches as targets and tools Contains sections on such topics as constructing and optimizing artificial riboswitches, synthetic biology: live cell imaging and intracellular sensors with artificial riboswitches, synthetic biology: conditional control of gene expression with artificial riboswitches, synthetic biology: using artificial riboswitches for protein evolution and pathway optimization, anti-riboswitches drug screens




[PDF] Proceedings Of The Seventh International Colloquium On Paratuberculosis

Proceedings of the Seventh International Colloquium on Paratuberculosis PDF
Author: International Association for Paratuberculosis
Publisher: International Assn for Paratuberculosis Incorporated
ISBN:
Size: 53.26 MB
Format: PDF, Docs
Category : Medical
Languages : en
Pages : 554
View: 4039

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Proceedings Of The Seventh International Colloquium On Paratuberculosis

by International Association for Paratuberculosis, release date 2003, Proceedings Of The Seventh International Colloquium On Paratuberculosis Books available in PDF, EPUB, Mobi Format. Download Proceedings Of The Seventh International Colloquium On Paratuberculosis books,




[PDF] 2 D Proteome Analysis Protocols

2 d Proteome Analysis Protocols PDF
Author: Andrew J. Link
Publisher: Springer Science & Business Media
ISBN: 1592595847
Size: 80.11 MB
Format: PDF, ePub, Docs
Category : Science
Languages : en
Pages : 601
View: 1934

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2 D Proteome Analysis Protocols

by Andrew J. Link, release date 1999, 2 D Proteome Analysis Protocols Books available in PDF, EPUB, Mobi Format. Download 2 D Proteome Analysis Protocols books, A step-by-step tour through the complete process of doing proteomics. With easy-to-follow instructions, complete with many helpful hints and explanations, leading investigators and pioneers in the field show how to make protein extracts, reproducibly run them on 2-D gels, detect them, analyze the data, and precisley identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the second dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains. 2-D Proteome Analysis Protocols is the most complete guide for using proteomics to answer biological questions.




[PDF] Antibiotic Resistance Protocols

Antibiotic Resistance Protocols PDF
Author: Stephen H. Gillespie
Publisher: Humana
ISBN:
Size: 77.15 MB
Format: PDF, Kindle
Category : Medical
Languages : en
Pages : 227
View: 1862

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Antibiotic Resistance Protocols

by Stephen H. Gillespie, release date 2010-04-16, Antibiotic Resistance Protocols Books available in PDF, EPUB, Mobi Format. Download Antibiotic Resistance Protocols books, This book fully updates and builds upon its first edition. Beginning with chapters on epidemiology and population genetics, it continues with sections covering genomics and gene expressions, fitness mutation and physiology, and the detection of resistance.




[PDF] Fems Microbiology Letters

FEMS Microbiology Letters PDF
Author:
Publisher:
ISBN:
Size: 35.77 MB
Format: PDF, Kindle
Category : Microbiology
Languages : en
Pages :
View: 4552

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Fems Microbiology Letters

by , release date 2001, Fems Microbiology Letters Books available in PDF, EPUB, Mobi Format. Download Fems Microbiology Letters books,